Greg Singer (Human Cancer Genetics, The Ohio State University)
(June 15, 2006 1:00 PM - 2:00 PM)
It is now known that the majority of human genes have more than one promoter. In other words, the process of transcription can initiate at more than one place in the gene, and each of these locations has its own regulatory mechanisms including unique transcription factor binding sites. If we are to truly understand gene expression in different tissues and diseases, we must investigate the activity of individual promoters within the gene. To do this, we are creating a custom microarray in which the probes are specifically designed to measure the activity of alternative promoters in human genes. I will discuss the approach we're taking in the design of these probes.