MBI Videos

Videos by Workshop 6: Transport in a cell

  • The Function of Dynein in Budding Yeast: Mechanism, Regulation and Checkpoint Activity
    John Cooper
    I will discuss work from our lab and other labs on how dynein functions to position the mitotic spindle and the nucleus in budding yeast. Interactions of cytoplasmic microtubules with the cortex control spindle position, and dynein represents one of the major pathways by which spindle position is controlled. Dynein functions by an "offloading" mechanism in which ...
  • The conserved L5 loop establishes the pre-powerstroke conformation of the kinesin-5 motor, Eg5
    Sarah Rice
    Kinesin superfamily motor proteins contain a structurally conserved loop near the ATP binding site, termed L5. The function of L5 is unknown, although several drug inhibitors of the mitotic kinesin Eg5 bind to L5. We used electron paramagnetic resonance spectroscopy (EPR) to investigate the function of L5 in Eg5. We site-specifically attached EPR probes to ADP, to L5, and ...
  • Mechanics of Actomyosin Interaction and the Role of Substrate Stiffness on Actin Network Dynamics
    Sean Sun
    Myosin is a major molecular force generator in the cell. The essential features of myosin interaction with actin filaments are understood. In the cell, the interactions of myosin with F-actin, substrate adhesions and other actin-associated proteins are less clear. We will borrow ideas from mathematical models of skeletal muscle to develop simple models for integrin focal a...
  • Road signs for kinesin transport
    Kristen Verhey
    We probe the transport properties in protein solutions stable with respect to any, solid or liquid, phase separation as a step in the understanding of transport in the cytosol of live cells. We determine the mean squared displacement of probe particles in the time range 1 millisecond - 10 seconds in solutions of a model protein. The tested solutions exhibit significant ela...
  • Motoring along a nucleic acid strand: template-dictated polymerization of macromolecules of life
    Debashish Chowdhury
    Polymerases and ribosome are molecular machines which perform three important biological functions. Like cytoskeletal motors, each of these moves along a track using chemical energy for performing mechanical work. Moreover, it decodes genetic information chemically-encoded in the sequence of the subunits of the track. Furthermore, it polymerizes a macromolecule (DNA, RNA o...
  • DNA chasing DNA: physics of homology recognition and the secret of perfect match
    Alexei Kornyshev
    DNA chasing DNA: physics of homology recognition and the secret of perfect match...

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